• Users Online: 235
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2016  |  Volume : 28  |  Issue : 2  |  Page : 66-70

Speciation of Candida isolates obtained from diarrheal stool


Department of Microbiology, Maulana Azad Medical College and Associated Hospitals, New Delhi, India

Correspondence Address:
Pragyan S Panda
Department of Microbiology, Maulana Azad Medical College and Associated Hospitals, New Delhi, 110002
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1110-7782.193888

Rights and Permissions

Introduction Candida is the most common fungi causing diarrhea. As pathogenesis and susceptibility to antifungals might vary in different species of Candida, their speciation is essential. Objective The aim of this study was to identify different species of Candida and investigate their antifungal susceptibility pattern. Materials and methods Of 2036 diarrheal stool samples, 212 samples were microscopically positive for budding yeast cells. We selected 50 of these samples and cultured on blood agar and MacConkey agar and incubated at 37°C for 18–24 h. Tiny colonies confirmed by means of Gram staining as budding yeast cells were subcultured on Sabouraud’s dextrose agar. Speciation of Candida was carried out using the germ tube test and inoculation into chrome agar and corn meal agar. Antifungal susceptibility testing was carried out as per CLSI guidelines using voriconazole, fluconazole, itraconazole, and amphotericin B. Results Candida was mostly isolated from children younger than 12 years (50%) and from male patients. A total of 64 isolates were obtained. Candida krusei (52%) was the most common isolate, followed by Candida tropicalis (16%), Candida albicans (0.02%), and Candida parapsilosis (0.01%). The isolates were mostly susceptible to voriconazole (97.4%), itraconazole (79.4%), amphotericin B (66.6%), and fluconazole (18%). Conclusion Candida diarrhea was mostly seen in individuals younger than 12 years, most commonly caused by C. krusei. Resistance to fluconazole was high. A rising resistance to amphotericin B is alarming. Speciation of Candida is important to see the difference in antifungal susceptibility in different species.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1040    
    Printed10    
    Emailed0    
    PDF Downloaded124    
    Comments [Add]    

Recommend this journal