|Year : 2019 | Volume
| Issue : 4 | Page : 397-402
A review of bone marrow niche cellular spectrum
Sabry A Shoieb1, Mohammad A Abdel Hafez1, Alaa E Abd El-Hamid1, Walaa Hamdy Ezz2
1 Department of Internal Medicine, Faculty of Medicine, Menoufia University, Menoufia, Egypt
2 Department of Nephrology, Zifta General Hospital, Gharbeya, Egypt
|Date of Submission||23-Apr-2019|
|Date of Acceptance||30-May-2019|
|Date of Web Publication||18-Aug-2020|
MBBCh Walaa Hamdy Ezz
El Monshat El Kubra, Markaz El Santa, Elgharbia, 31758
Source of Support: None, Conflict of Interest: None
To perform a narrative review on the role of bone marrow (BM) niche in normal hematopoiesis. MEDLINE databases and Ovid database were searched. The search was performed on 10 October 2018 and included articles published from 2015 to 2018 in English language. The initial search presented 45 articles, which were included in the study. Hematopoietic stem cells which produce a variety of hematopoietic lineage cells throughout the life are located in a specialized microenvironment called the ‘niche’ in the BM where they are governed. Several types of cells in the BM have been suggested to contribute to hematopoietic stem cell niche activity.
Keywords: bone marrow niche, hematopoiesis, hematopoietic stem cells
|How to cite this article:|
Shoieb SA, Abdel Hafez MA, Abd El-Hamid AE, Ezz WH. A review of bone marrow niche cellular spectrum. Egypt J Intern Med 2019;31:397-402
|How to cite this URL:|
Shoieb SA, Abdel Hafez MA, Abd El-Hamid AE, Ezz WH. A review of bone marrow niche cellular spectrum. Egypt J Intern Med [serial online] 2019 [cited 2020 Sep 29];31:397-402. Available from: http://www.esim.eg.net/text.asp?2019/31/4/397/292247
| Introduction|| |
For many years, the bone marrow (BM) niche was considered to be little more than an inert scaffold, providing structure for the much more interesting hematopoietic stem and progenitor cell activity contained within. In the past decade, however, the BM microenvironment has been recognized to be a vibrant living tissue that, in addition to important homeostatic roles in hematopoiesis, can aid neoplastic disease processes .
The niche hypothesis was proposed by Ray Schofield in 1978. By noticing that CFU-S ‘stem cells’ have less robust reconstitution capacity in irradiated mice compared with cells from BM, he hypothesized the existence of a BM niche that preserves stem cells .
An intricate and dynamic relationship between hematopoietic stem cell (HSC) ‘seeds’ and the niche ‘soil’ helps to determine whether the product of hematopoiesis will be a healthy, useful mature blood cells or instead an overgrowth of developmentally stunted, immature, dysfunctional dysplastic blasts − akin to hematological ‘weeds’ .
The interest in the study of the HSCs niche is accurate characterization of the cell populations and factors produced by them, responsible for HSCs maintenance in vivo .
| The stem cell niche spectrum|| |
The anatomy of the BM may unravel the specific niches where HSCs live and are regulated .
Cells of the osteoblastic lineage have been proposed to function as significant modulators of HSCs in the BM. In vitro culture experiments have suggested that osteoblastic cell lines can expand the number of HSCs two fold to four fold . Additionally, when cotransplanted with HSCs, osteoblasts can increase the engraftment rate. Osteoblasts secrete a wide group of growth factors and cytokines, important for HSC maturation. Osteopontin, an osteoblast-secreted protein, contributes to HSC location and is a negative regulator of their proliferation .
Furthermore, angiopoietin-1 expressed by osteoblasts has been proposed to regulate HSC numbers through the activation of the Tie-2 signaling pathway, whereas Jagged 1, a Notch receptor ligand, supports an increase in HSC numbers .
Osteocytes, derived from osteoblasts that become embedded within the bone matrix, appear to have an inhibitory effect on HSC support .
It had been shown that cells of the osteoblastic lineage may support lymphopoiesis. Thus, the precise function of osteoblasts in hematopoiesis remains indistinct; subsequent studies indicate that they are dispensable for HSC maintenance and more important to lymphoid progenitors.
Vascular endothelial cells isolated from BM are known to promote the proliferation and differentiation of human CD34+ progenitor cells in vitro .
Blocking angiogenic activity of endothelial cells by neutralizing vascular endothelial-cadherin and vascular endothelial growth factor receptor-2 impaired supportive function of endothelial cells to long-term HSCs .
Despite the relatively small amount of niche factor expression, conditional deletion of niche factors, CXL12 or SCF, from endothelial cells led to the decrease of HSC number in BM, suggesting that endothelial cells play indispensable roles for HSC maintenance through producing these niche factors .
Endothelial cells have also been shown to regulate HSC quiescence through surface molecule E-selectin expression. Deletion or blockade of E-selectin promoted HSC quiescence and increased survival through undefined mechanism .
Although the heterogeneity of endothelial cell populations remains largely unresolved, endothelial cells with a high expression of CD31 and endomucin (Emcn), referred to as type H endothelium, which are found in end-terminal arterioles connecting to sinusoids, expressed Kitl-encoding stem cell factor at higher levels than sinusoidal type L endothelial cells .
A differential role of endothelial cells is reflected by the difference of vascular permeability observed between sinusoids and arterioles which has repercussions on HSC egress and homing. Arterial vessels are less permeable and maintain HSCs in a low reactive oxygen species, keeping HSCs in a quiescent state. On the contrary, more leaky sinusoids expose HSCs to blood plasma and promote high level of ROS in HSCs, augmenting the ability of differentiation and migration .
Pericytes have long projections that surround the blood vessel wall in almost all tissues . They are defined by their anatomical locations in combination with several molecular markers such as platelet-derived growth factor receptor β, CD146, neuron-glial 2, SMA, desmin, and nestin. However, marker expression is highly dependent on the tissue type and could often be affected by the pathologic state of the organ .
Interestingly, an evaluation of the cell cycle status demonstrated that arteriolar pericytes are largely quiescent. Tridimensional imaging of the adult mouse BM has revealed that the majority of dormant HSCs are located close to arterioles, and genetic depletion of arteriolar pericytes resulted in migration of HSCs away from the arterioles, switching them into nonquiescent status. This proposes that arteriolar pericytes promote HSC dormancy, essential for HSC maintenance in the BM . Nevertheless, the molecular mechanisms by which arteriolar pericytes regulate HSC quiescence remain to be clearly defined.
Schwann cells are the main glial cells of the peripheral nervous system. In the BM, they are present in their unmyelinated form associated with sympathetic and sensory nerve fibers . Unmyelinated Schwann cells received relatively little scientific attention, with only a poor understanding having developed of their function outside the nervous system. Schwann cells maintain the ability to revert to an immature phenotype in response to injury and disease, and by doing so, they can then re-enter the cell cycle, proliferate, and affect the microenvironment in which they are located .
Schwann cells were shown to produce several cytokines and to express cytokine receptors as well . Studies have suggested that BM Schwann cells regulate the hibernation and activation of HSCs. In a study, using immunohistochemistry, the expression of active transforming growth factor-beta (TGF-β) was suggested to be confined to BM Schwann cells. BM denervation reduced the number of cells producing active TGF-β, leading to a loss of HSCs from the BM .
However, denervation does not only affect Schwann cells; for instance, β-adrenergic signals from the sympathetic nervous system have been shown to regulate enforced and circadian HSC egress . Moreover, in addition to Schwann cells, other cells, including megakaryocytes, produce TGF-β in the BM. TGF-β1 derived from megakaryocytes has been shown to maintain HSC quiescence. Most of BM innervation − and Schwann cell ensheathing of those nerve fibers − runs along arterioles in the BM .
As arterioles contain several cell types, the identity of the cell promoting HSC quiescence is still unknown. It will be interesting to ascertain whether BM Schwann cells differ from Schwann cells from other tissues. Successful isolation of BM Schwann cells may enable the discovery of novel niche factors possibly secreted by those cells.
Signals from the sympathetic nervous system have been identified as regulatory components of the HSC niche . Sympathetic nerves secrete catecholamines, which are delivered to the BM microenvironment by the blood circulation or by secretion from the nerve endings acting in paracrine signaling .
It has been shown that trafficking of HSCs into the bloodstream during steady state is strongly regulated through the circadian release of adrenergic signals from the sympathetic nerves in the BM .
Adrenergic signaling reduces CXCL12 expression in the BM . Moreover, evidence shows that chemotherapy-induced injury of sympathetic nerves in the BM prevents hematopoietic recovery, suggesting that treatment with neuroprotective drugs during chemotherapy would preserve HSC function in the BM niche. Induction of sympathetic neuropathy by malignant cells has been demonstrated .
HSCs express catecholaminergic receptors, suggesting that they are able to directly respond to signals from the sympathetic nervous system. Treatment of HSCs with dopamine agonists augments colony-formation in vitro, albeit only in the presence of granulocyte-colony stimulating factor (G-CSF). Pretreatment of HSCs with dopamine agonists augments their ability to engraft. Additionally, norepinephrine treatment of HSCs also enhances both colony-formation capabilities in vitro and engraftment in vivo .
Additionally, the sympathetic nervous system adjacent to the dorsal aorta plays a vital role in HSC specification during development .
Osteoclasts are the only cell type capable of bone resorption in the human body, allowing the renewal of the skeleton but also opening space in the BM for hematopoietic cells. Osteoclasts have been suggested to contribute to HSC release via enzyme secretion, which enhances mobilization .
On the contrary, it has been hypothesized that osteoclastic bone resorption releases calcium, increasing its concentration at the endosteal region, which attracts and retains HSCs that express calcium-sensing receptors in the BM. Bone resorption also produces active TGF-β, which can act on HSCs .
Osteoclast inhibition by bisphosphonates causes a reduction in the number of HSCs and delays hematopoietic recovery. Using a mouse model with loss of osteoclast activity, osteoclasts have been shown to regulate mesenchymal cell differentiation and HSC maintenance .
Macrophages play diverse roles in the bone and marrow. At the sites of bone remodeling, they are anatomically juxtaposed with endosteal osteoblasts and participate in bone mineralization .
Radio-resistant macrophages protect the HSC pool from exhaustion by producing prostaglandin E2 after irradiation . BM-resident macrophages are defined based on differential expression of numerous molecular markers, such as Gr-1, F4/80, CD115, and CD169 .
Their numbers are reduced during G-CSF-induced HSC mobilization, and following their loss, HSCs egress to the peripheral circulation . Additionally, a separate subpopulation of macrophages expressing high levels of smooth muscle actin and cyclooxygenase 2 was recently identified. This rare macrophage population synthesizes prostaglandin E2, which increases CXCL12 expression in nestin-GFP (Green Fluorescent Protein)+mesenchymal stem cells  and CXCR4 expression on HSCs, thus improving the survival and maintenance of HSCs in the BM. Moreover, macrophages have been suggested to regulate HSC egress from the BM after phagocytosis of aged neutrophils .
Macrophages promote HSC retention in the BM by regulating the expression of CXCL12 by nestin-GFP+MSCs via a soluble factor secreted by CD169+macrophages. Subsequent studies have suggested that this factor is oncostatin M .
Thus, macrophages and sympathetic nerves exert the opposite action to the microenvironment, forming a regulatory loop. HSC retention in the BM and the spleen relies at least partially on a ligand for vascular cell adhesion molecule 1, integrin VLA-4 , which is expressed by macrophages. A subsequent study described that macrophages are important players in splenic HSC retention, as depleting macrophages using inducible diphtheria toxin receptor expression at the CD169 locus or silencing VCAM-1 in macrophages caused release of HSCs from the spleen.
Interestingly, macrophages are also key mediators of the neuroprotective effect of neuropeptide Y, and thus contribute to HSC survival in the BM. Neuropeptide Y regulates homeostasis in several tissues through Y receptors .
It had been shown that neuropeptide Y deficiency impairs HSC survival and BM regeneration. Furthermore, pharmacological elevation of neuropeptide Y prevented the deficits, whereas neuropeptide Y injection into mice lacking the Y1 receptor specifically in macrophages did not rescue BM dysfunction .
Macrophage depletion also delays erythropoietic recovery following acute blood loss, myelo-ablation, or challenge with hemoglobin-oxidizing phenylhydrazine. Interestingly, polycythemia vera, which is characterized by increased erythropoiesis, can be improved via macrophage ablation. Macrophage depletion reduces hematocrit and red blood cell counts in a mouse model of polycythemia vera driven by the JAK2V617F mutation. These studies suggest that macrophages are crucial components of erythroid maturation in the steady state, as well as during erythropoietic rescue after stress and disease. It remains unknown how erythroblastic island macrophages differ functionally from other BM-resident macrophages. Erythroblastic island macrophages are thought to be very large, express F4/80, and do not express Mac1 ; however, a unique molecular marker for erythroblastic island macrophages remains to be discovered. Overall, these findings suggest that macrophages are key components of the BM promoting the maintenance and retention of HSCs.
A subset of HSCs is located near megakaryocytes in the BM .
Megakaryocytes produce multiple cytokines (e.g. thrombopoietin, TGF-β, and CXCL4); it is possible that their effect on HSCs results from the balance of all those and probably more molecules. Interestingly, megakaryocytes physically associate with ∼20% of HSCs in the BM. Megakaryocytes serve as HSC-derived niche cells directly regulating HSC function .
Thus, some studies have suggested a complex interaction between megakaryocytes, HSCs, and the osteolineage within the BM. Accordingly, after BM radioablation, host megakaryocytes are recruited to the endosteum, where osteoblasts undergo rapid expansion in response to the secretion of megakaryocyte-derived mesenchymal growth factors, such as platelet-derived growth factor-β to promote HSC engraftment and hematopoietic reconstitution after BM transplantation .
The migration of megakaryocytes to the endosteum is thought to depend on thrombopoietin signaling, as the inhibition of c-Mpl reduces megakaryocyte migration after radioablative conditioning. More findings have identified a direct HSC regulation by megakaryocytes in steady-state hematopoiesis. Ablation of megakaryocytes reduces HSC engraftment and proliferation .
Thrombopoietin administration to megakaryocyte-depleted mice restores the number of quiescent HSCs , suggesting that thrombopoietin may contribute to regulation of HSCs by megakaryocytes.
Megakaryocytes secrete high levels of TGF-β, which regulates HSCs. Conditional deletion of TGF-β1 in megakaryocytes increases HSC activation and proliferation in young mice. In addition, TGF-β injection into megakaryocyte-depleted mice restores HSC quiescence .
Under homeostatic conditions, megakaryocytes maintain HSC quiescence through TGF-β signaling, whereas under stress, megakaryocytes promote HSC expansion via fibroblast growth factor 1 production. CXCL4, which is produced by megakaryocytes, negatively regulates HSC proliferation, reduces HSCs numbers, and decreases engraftment. An increase in HSC number, proliferation, and repopulating activity was observed in CXCL4 knockout mice .
Lymphocytes, essential for both cell-mediated and antibody-mediated immunity, are broadly distributed throughout the BM parenchyma and make up a major fraction of total BM mononuclear cells . Lymphocytes have been suggested to influence hematopoiesis, potentially through direct cellular interactions with the HSCs. Natural killer cells have been suggested to play a negative role in HSC differentiation .
Regulatory T cells make up one-third of all CD4+ T cells in the BM . Depletion experiments and cotransfer of BM with regulatory T cells indicated that these cells suppress colony formation and myeloid differentiation of HSCs .
Moreover, FoxP3+ regulatory T cells colocalize with HSCs in the endosteal surface in the calvarial and trabecular BM, and this colocalization is lost after depletion of regulatory T cells. However, whether this interaction is biologically relevant to homeostatic HSCs maintenance is still unclear. Furthermore, regulatory T cells have been suggested to provide an immune-privileged microenvironment in the BM, protecting HSCs from immune attacks .
Neutrophils are the most abundant subpopulation of leukocytes. Neutrophils have a short circulating half-life (6–8 h), after which they quickly migrate to tissues where they perform their functions .
Serine proteases derived from neutrophils are capable of cleaving several cytokines and receptors essential for HSCs retention in vitro, including CXCL12, CXCR4, VCAM-1, c-Kit, and SCF, suggesting that activated neutrophils create a proteolytic niche that may contribute to HSCs release from the BM. However, it was shown that, in mice lacking these proteases, G-CSF-induced HSC mobilization proceeds normally , suggesting that serine proteases are not essential for BM egress. G-CSF induces neutrophil expansion in the BM, which may lead to mesenchymal stem cell and osteoblast apoptosis and reductions in the expression of factors that are responsible for HSC retention in the BM. However, neutrophil number in the BM does not certainly correlate with HSCs mobilization .
Those effects are lost in mice in which neutrophils do not express CXCR4 and lack tropism to the BM, indicating that these effects may be exerted locally in the BM . Ablation of BM macrophages reverses the niche-modulating functions of neutrophils, indicating that the effect of neutrophils on the hematopoietic niche is dependent on macrophages. Together, these data explain how the daily clearance of aged neutrophils in the BM generates signals that affect HSCs in the BM niches .
| Conclusion|| |
In the homeostasis state, niche precisely controls the HSC fate decisions and adjusts a balance between the rate of HSC self-renewal and differentiation. Mobilization and homing of HSCs are important physiological processes and are highly controlled by niche elements.
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Conflicts of interestThere are no conflicts of interest
| References|| |
Cogle CR, Saki N, Khodadi E, Li J, Shahjahani M, Azizodoost S. Bone marrow niche in the myelodysplastic syndromes. Leuk Res 2015; 39:1020–1027.
Schofield R. The relationship between the spleen colony-forming cell and the haemopoietic stem cell. Blood Cells 1978; 4:7–25
Raza A, Cruz R, Latif T, Mukherjee S, Galili N. The biology of myelodysplastic syndromes: unity despite heterogeneity. Hematol Rep 2010; 2:e4.
Bulycheva E, Rauner M, Medyouf H, Theorl I, Bornhauser M, Hofbauer LC et al.
Myelodysplasia is in the niche: novel concepts and emerging therapies. Leukemia 2015; 29:259–268.
Ellis SL, Grassinger J, Jones A, Judy B, Camenisch T, Haylock D et al.
The relationship between bone, hemopoietic stem cells, and vasculature. Blood 2011; 118:1516–1524.
Taichman RS, Emerson SG. The role of osteoblasts in the hematopoietic microenvironment. Stem cells 1998; 16:7–15.
Nilsson SK, Johnston HM, Whitty GA, Williams B, Webb RJ, Denhardt DT et al.
Osteopontin, a key component of the HSC niche and regulator of primitive hematopoietic progenitor cells. Blood 2005; 106:1232–1239.
Calvi LM, Adams GB, Weibrecht KW, Weber JM, Olson DP, Knight MC et al.
Osteoblastic cells regulate the haematopoietic stem cell niche. Nature 2003; 425:841–846.
Hooper AT, Butler JM, Nolan DJ, Kranz A, Iida K, Kobayashi M et al.
Engraftment and reconstitution of hematopoiesis is dependent on VEGFR2-mediated regeneration of sinusoidal endothelial cells. Cell Stem Cell 2009; 4:263–274.
Rafii S, Shapiro F, Pettengell R, Ferris B, Nachman RL, Moore MA, Asch AS. Human BM microvascular endo-thelial cells support long-term proliferation and differentiation of myeloid and megakaryocytic progenitors. Blood 1995; 86:3353–3363.
Butler JM, Nolan DJ, Vertes EL, Varnum-Finney B, Kobayashi H, Hooper AT et al.
Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent HSCs. Cell Stem Cell 2010; 6:251–264.
Ding L, Saunders TL, Enikolopov G, Morrison SJ. Endothelial and perivascular cells maintain haematopoietic stem cells. Nature 2012; 481:457–462.
Winkler IG, Barbier V, Nowlan B, Jacobsen RN, Forristal CE, Patton JT et al.
Vascular niche E-selectin regulates HSC dormancy, self-renewal and chemoresistance. Nat Med 2012; 18:1651–1657.
Kusumbe AP, Ramasamy SK, Itkin T, Mae MA, Langen UH, Betsholtz C et al.
Age-dependent modulation of vascular niches for haematopoietic stem cells. Nature 2016; 532:380–384.
Itkin T, Gur-Cohen S, Spencer JA, Schajnovitz A, Ramasamy SK, Kusumbe AP et al.
Distinct BM blood vessels differentially regulate haematopoiesis. Nature 2016; 532:323–328.
Birbrair A, Delbono O. Pericytes are essential for skeletal muscle formation. Stem Cell Rev 2015; 11:547–548.
Mendez-Ferrer S, Michurina TV, Ferraro F, Mazloom AR, Macarthur BD, Lira SA et al.
Mesenchymal and haematopoietic stem cells form a unique BM niche. Nature 2010; 466:829–834.
Son YJ, Thompson WJ. Schwann cell processes guide regeneration of peripheral axons. Neuron 1995; 14:125–132.
Ozaki A, Nagai A, Lee YB, Myong NH, Kim SU. Expression of cytokines and cytokine receptors in human Schwann cells. Neuroreport 2008; 19:31–35.
Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S et al.
Non-myelinating Schwann cells maintain HSC hibernation in the BM niche. Cell 2011; 147:1146–1158.
Mendez-Ferrer S, Lucas D, Battista M, Frenette PS. Haematopoietic stem cell release is regulated by circadian oscillations. Nature, 2008; 452:442–447.
Yamazaki K, Allen TD. Ultrastructural morphometric study of efferent nerve terminals on murine BM stromal cells, and the recognition of a novel anatomical unit: the ‘neuro-reticular complex’. Am J Anat 1990; 187:261–276.
Mendelson A, Frenette PS. Hematopoietic stem cell niche maintenance during homeostasis and regeneration. Nat Med 2014; 20:833–846.
Afan AM, Broome CS, Nicholls SE, Whetton AD, Miyan JA. Bone marrow innervation regulates cellular retention in the murine haemopoietic system. Br J Haematol 1997; 98:569–577.
Lucas D, Battista M, Shi PA, Isola L, Frenette PS. Mobilized HSC yield depends on species-specific circadian timing. Cell Stem Cell 2008; 3:364–366.
Hanoun M, Zhang D, Mizoguchi T, Pinho S, Pierce H, Kunisaki Y, Lacombe J et al.
Acute myelogenous leukemia-induced sympathetic neuropathy promotes malignancy in an altered HSC niche. Cell Stem Cell 2014; 15:365–375.
Spiegel A, Shivtiel S, Kalinkovich A, Ludin A, Netzer N, Goichberg P et al.
Catecholaminergic neurotransmitters regulate migration and repopulation of immature human CD34+ cells through Wnt signaling. Nat Immunol 2007; 8:1123–1131.
Fitch SR, Kimber GM, Wilson NK, Parker A, Mirshekar-Syahkal B, Göttgens B et al.
Signaling from the sympathetic nervous system regulates HSC emergence during embryogenesis. Cell Stem Cell 2012; 11:554–566.
Kollet O, Dar A, Shivtiel S, Kalinkovich A, Lapid K, Sztainberg Y et al.
Osteoclasts degrade endosteal components and promote mobilization of hematopoietic progenitor cells. Nat Med 2006; 12:657–664.
Balooch G, Balooch M, Nalla RK, Schilling S, Filvaroff EH, Marshall GW et al.
TG regulates the mechanical properties and composition of bone matrix. Proc Natl Acad Sci USA 2005; 102:18813–18818.
Mansour A, Abou-Ezzi G, Sitnicka E, Jacobsen SE, Wakkach A, Blin-Wakkach C. Osteoclasts promote the formation of HSC niches in the BM. J Exp Med 2012; 209:537–549.
Alexander KA, Chang MK, Maylin ER, Kohler T, Müller R, Wu AC et al.
Osteal macrophages promote in vivo intramembranous bone healing in a mouse tibial injury model. J Bone Miner Res 2011; 26:1517–1532.
Winkler IG, Sims NA, Pettit AR, Barbier V, Nowlan B, Helwani F et al.
Bone marrow macrophages maintain hematopoietic stem cell (HSC) niches and their depletion mobilizes HSCs. Blood 2010; 116:4815–4828.
Chow A, Lucas D, Hidalgo A, Méndez-Ferrer S, Hashimoto D, Scheiermann C et al.
Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J Exp Med 2011; 208:261–271.
Ludin A, Itkin T, Gur-Cohen S, Mildner A, Shezen E, Golan K et al.
Monocytes-macrophages that express smooth muscle actin preserve primitive hematopoietic cells in the BM. Nat Immunol 2012; 13:1072–1082.
Casanova-Acebes M, Pitaval C, Weiss LA, Nombela-Arrieta C, Chèvre R, A-González N et al.
Rhythmic modulation of the hematopoietic niche through neutrophil clearance. Cell 2013; 153:1025–1035.
Albiero M, Poncina N, Ciciliot S, Cappellari R, Menegazzo L, Ferraro F et al.
Bone marrow macrophages contribute to diabetic stem cell mobilopathy by producing oncostatin M. Diabetes 2015; 64:2957–2968.
Williams DA, Rios M, Stephens C, Patel VP. Fibronectin and VLA-4 in haematopoietic stem cell microenvironment interactions. Nature 1991; 352:438–441.
Wheway J, Mackay CR, Newton RA, Sainsbury A, Boey D, Herzog H, Mackay F. A fundamental bimodal role for neuropeptide Y1 receptor in the immune system. J ExpMed 2005; 202:1527–1538.
Park MH, Jin HK, Min WK, Lee WW, Lee JE, Akiyama H et al.
Neuropeptide Y regulates the HSC microenvironment and prevents nerve injury in the BM. EMBO J 2015; 34:1648–1660.
Manwani D, Bieker JJ. The erythroblastic island. CurrTopDevBiol 2008; 82:23–53.
Nakamura-Ishizu A, Takubo K, Fujioka M, Suda T. Megakaryocytes are essential for HSC quiescence through the production of thrombopoietin. Biochem Biophys Res Commun 2014; 454:353–357.
Birbrair A, Frenette PS. Niche heterogeneity in the BM. Ann NY Acad Sci 2016; 1370:82–96.
Olson TS, Caselli A, Otsuru S, Hofmann TJ, Williams R, Paolucci P et al.
Megakaryocytes promote murine osteoblastic HSC niche expansion and stem cell engraftment after radioablative conditioning. Blood, 2013; 121:5238–5249.
Zhao M, Perry JM, Marshall H, Venkatraman A, Qian P, He XC et al.
Megakaryocytes maintain homeostatic quiescence and promote post-injury regeneration of HSCs. Nat Med 2014; 20:1321–1326.
Bruns I, Lucas D, Pinho S, Ahmed J, Lambert MP, Kunisaki Y et al.
Megakaryocytes regulate HSC quiescence through CXCL4 secretion. Nat Med 2014; 20:1315–1320.
Mercier FE, Ragu C, Scadden DT. The BM at the crossroads of blood and immunity. Nat Rev Immunol 2012; 12:49–60.
Degliantoni G, Murphy M, Kobayashi M, Francis MK, Perussia B, Trinchieri G. Natural killer (NK) cell-derived hematopoietic colony-inhibiting activity and NK cytotoxic factor. Relationship with tumor necrosis factor and synergism with immune interferon. J Exp Med 1985; 162:1512–1530.
Zeng D, Hoffmann P, Lan F, Huie P, Higgins J, Strober S. Unique patterns of surface receptors, cytokine secretion, and immune functions distinguish T cells in the BM from those in the periphery: impact on allogeneic BM transplantation. Blood 2002; 99:1449–1457.
Urbieta M, Barao I, Jones M, Jurecic R, Panoskaltsis-Mortari A, Blazar BR et al.
Hematopoietic progenitor cell regulation by CD4+CD25+ T cells. Blood 2010; 115:4934–4943.
Fujisaki J, Wu J, Carlson AL, Silberstein L, Putheti P, Larocca R et al.
In vivo imaging of T reg cells providing immune privilege to the haematopoietic stem-cell niche. Nature 2011; 474:216–219.
Summers C, Rankin SM, Condliffe AM, Singh N, Peters AM, Chilvers ER. Neutrophil kinetics in health and disease. Trends Immunol 2010; 31:318–324.
Levesque JP, Liu F, Simmons PJ, Betsuyaku T, Senior RM, Pham C et al.
Characterization of hematopoietic progenitor mobilization in protease-deficient mice. Blood 2004; 104:65–72.
Christopher MJ, Rao M, Liu F, Woloszynek JR, Link DC. Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice. J Exp Med 2011; 208:251–260.
Eash KJ, Means JM, White DW, Link DC. CXCR4 is a key regulator of neutrophil release from the BM under basal and stress granulopoiesis conditions. Blood 2009; 113:4711–4719.